Dot-Blot Enzyme Immunoassay for the Detection of Bovine Herpes Virus-1(BHV-1) Antibodies
نویسندگان
چکیده
Various detection methods have been developed for serological monitoring BHV1, an alphaherpesvirus and the cause of the respiratory disease, abortion, conjectivitis and other clinical forms of disease complex in cattle, in different laboratories throughout the world. The objective of this study was to develop and evaluate dot-blot enzyme immunoassay as a proper and inexpensive method for detection of BHV-1 antibodies in cattle. In this assay, a whole particle of a filed isolated of BHV-1, identified by PCR and VNT and was used as an antigen. A total of 360 bovine sera in three different age groups with known ELISA titer for BHV-1 antibodies and rabbit anti cow Ig HRP conjugated (Dako, Denmark) were used as primary and secondary antibody, respectively.4-chloronaphtole (Merk, Germany) was used as substrate. The concentrations of HRP-conjugated, sera and antigen were optimized. Statistical analysis showed sensitivity, specificity, Positive Predictive Value (PPV) and Negative Predictive Value (NPV) of Dot-blot enzyme immunoassay were 86.4%, 92.7%, 94.4% and 84.4% respectively.
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